isotype control  (Bio-Rad)

Journal: Bioactive Materials

Article Title: Immunomodulatory effects of biodegradable Mg–Cu–Zn alloy in esophageal cancer

doi: 10.1016/j.bioactmat.2026.02.046

Figure Lengend Snippet: Distribution of CD163 + M2 TAMs in AKR-derived allograft tumor tissues from immunocompetent C57BL/6 mice. (a, b) Representative IHC staining images showing CD163 + M2 TAMs in the (a) peritumoral stroma and (b) tumor islets. Lower panels display higher-magnification views of the regions outlined by red dashed boxes. (c, d) Quantification of CD163 + cells in the (c) peritumoral stroma and (d) tumor islets. (e) Comparison of CD163 + cell density between the peritumoral stroma and tumor islets. (f) Total number of CD163 + cells in allograft tumors (peritumoral stroma and tumor islets combined). (g, h) Comparison of the density between iNOS + cells and CD163 + cells in the (g) peritumoral stroma and (h) tumor islets. (i, j) Quantification of iNOS + /CD163 + ratio in the (i) peritumoral stroma and (j) tumor islets. p < 0.05 (∗), p < 0.01 (∗∗), p < 0.001 (∗∗∗). A field of view is ∼0.086 mm 2 in (c−j).

Article Snippet: Tissue sections were then incubated with primary antibodies against iNOS (22226-1-AP, ProteinTech, China), CD163 (A26411PM, Abclone, China), CD8 (SP16, Maixin, China), CD4 (SP35, Maixin, China) or Ki-67 (12202S, Cell Signaling Technology) for 12 h at 4 °C, followed by secondary antibodies (Beyotime Biotechnology, Nantong, China).

Techniques: Derivative Assay, Immunohistochemistry, Comparison

Journal: bioRxiv

Article Title: Single-cell characterization of tumor immune landscapes in colorectal cancer humanized mice

doi: 10.64898/2026.06.01.729295

Figure Lengend Snippet: (A) UMAP of CD45 + immune cells from HIS mouse tumors, split per CMS subtype (n = 14,578 cells). (B) Boxplots showing proportions of tumor-infiltrating immune cells across major lineages (T, NK, myeloid, and B cells) stratified by CMS subtype. (C) Representative immunohistochemical staining of FFPE sections from subcutaneous HIS CRC tumors of CMS1 and CMS4 subtypes using CD4, CD8, and CD163, with corresponding boxplot quantification of CD4 + , CD8 + , and CD163 + cells expressed as cells/mm . Statistical significance was determined using a two-tailed Mann–Whitney test. Only statistically significant differences are shown (**p ≤ 0.01; ***p ≤ 0.001). (D) Dotplot of genes associated with human macrophage characterization across myeloid subsets in HIS tumors. (E) Dotplot of genes associated with human T cell exhaustion across T cell subsets in HIS tumors. Dot size indicates the proportion of cells expressing the gene, and color indicates expression level. B: B cell; T: T cell; Tn: T naive; Tm: T memory; Treg: regulatory T; CD4Tfh: CD4 + follicular helper T cell; CD4Th17: CD4 + Th17 cell; MAIT: mucosal associated invariant T; NKT: natural killer T; dnT: double negative T; NK: natural killer cell; pDC: plasmacytoid dendritic cell; mono-macs: monocytes-macrophages; cDC1: conventional dendritic cell type 1; cDC2: conventional dendritic cell type 2; UMAP: Uniform Manifold Approximation and Projection; HIS: humanized immune system; CMS: Consensus Molecular Subtype.

Article Snippet: Primary antibodies CD4 (Abcam, 1:50), CD8 (DAKO, 1:50), CD20 (DAKO, 1:500), CD56 (Monosan, 1:50), CD163 (Cell Marque Corporation, 1:25) were diluted in antibody diluent (Agilent: CD4; Ventana: CD8, CD20, CD56, CD163) and incubated in a humidified chamber.

Techniques: Immunohistochemical staining, Staining, Two Tailed Test, MANN-WHITNEY, Expressing